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sufB [2019-07-10 10:32:46]
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sufB [2019-07-10 10:32:46]

FeS scaffold, synthesis of Fe-S-clusters
locus
BSU32670
pI
4.96
mw
52.56 kDa
protein length
465 aa Sequence Blast
gene length
1398 bp Sequence Blast
function
synthesis of iron-sulfur clusters
product
FeS scaffold
essential
yes
synonyms
yurU
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.3|Biosynthesis of iron-sulfur clusters]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    3,355,593 3,356,990

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • the mutant is viable and Fe-S clusters are dispensable if [protein|search|IspG ]and [protein|search|IspH ]from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate [pubmed|29292548]

    • The protein

    Catalyzed reaction/ biological activity

  • synthesis of Fe-S-clusters (the [protein|5991F051C5BAD61A0860BBC64A37DB9D16130998|SufB]-[protein|E966F3808222820C0644DC98F95765B89E6A2551|SufC]-[protein|1A9DA839AB77AF53958CB8426607BEF14F62B85E|SufD] complex) [pubmed|29292548]

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|5AWF] (from ''E. coli'', the [protein|5991F051C5BAD61A0860BBC64A37DB9D16130998|SufB]-[protein|E966F3808222820C0644DC98F95765B89E6A2551|SufC]-[protein|1A9DA839AB77AF53958CB8426607BEF14F62B85E|SufD] complex, 41% identity) [Pubmed|26472926]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|E966F3808222820C0644DC98F95765B89E6A2551|sufC]-[gene|1A9DA839AB77AF53958CB8426607BEF14F62B85E|sufD]-[gene|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]-[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]-[gene|5991F051C5BAD61A0860BBC64A37DB9D16130998|sufB]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B583 (yurU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1582 NBRP B. subtilis, Japan]

    • References

    Reviews

  • 28882201

    • Original publications

  • 17981983,20097860,22383849,15378759,26472926,29292548